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Image Search Results
Journal: Animals : an Open Access Journal from MDPI
Article Title: Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis
doi: 10.3390/ani12213026
Figure Lengend Snippet: List of primers employed for q-RT-PCR.
Article Snippet: The proteins were then transferred onto PVDF membranes (GE Bioscience, Newark, NJ, USA) and then blocked with 5% BSA (dissolved in TBST) for 1 h and the membrane was incubated with anti-SYCP3 (ab97672; Abcam, Cambridge, UK), anti-DDX4 (bs-22896R; Bioss Biotech, Beijing, China), anti-SOX9 (ab185966; Abcam, Cambridge, UK),
Techniques: Sequencing
Journal: Animals : an Open Access Journal from MDPI
Article Title: Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis
doi: 10.3390/ani12213026
Figure Lengend Snippet: Expressions of genes, proteins involved in ferroptosis in testis of mice in each group. ( A ) Nrf2 mRNA expression. ( B ) SLC7A11 mRNA expression. ( C ) GPX4 mRNA expression. ( D ) Western blot detection of SLC7A11, GPX4, and 4-HNE protein. ( E ) Image J analysis showing the grey value of SLC7A11. ( F ) Image J analysis showing the grey value of GPX4. ( G ) Image J analysis showing the grey value of 4-HNE. The data are expressed as means ± SEM, with n ≥ 3; Different lowercase letters (a, b, c, d) indicate significant differences ( p < 0.05).
Article Snippet: The proteins were then transferred onto PVDF membranes (GE Bioscience, Newark, NJ, USA) and then blocked with 5% BSA (dissolved in TBST) for 1 h and the membrane was incubated with anti-SYCP3 (ab97672; Abcam, Cambridge, UK), anti-DDX4 (bs-22896R; Bioss Biotech, Beijing, China), anti-SOX9 (ab185966; Abcam, Cambridge, UK),
Techniques: Expressing, Western Blot